In line with our results, these findings also showed the reproduction of antioxidant enzymes in heart homogenate after crocin administration. Determination of functional enzymes of liver and heart To measure the serum concentrations of hepatic transaminases and cardiac enzymes including creatine phosphokinase CPK and lactate dehydrogenase LDH ], serum was separated by blood centrifugation rpm, 5 min. These pathways include disturbance in homeostasis of calcium in cells, absence of ATP, activation of phospholipases and proteases and production of active oxygen and nitrogen species that lead to oxidative stress Promptly changing the liver color to a pallor shade confirmed the correctness of ischemia Thirty two rats were randomly assigned into 4 experimental groups 8 rats in each. Tissue samples of heart and blood were taken to use for further microscopic and laboratories analysis. Liver ischemia-reperfusion IR insult is an important side effect, which happens following liver graft, hapatectomy, and hepatic pedicle clamping during liver interventions 1.
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